The heat shock proteins were discovered, as the name suggests, since they are heavily upregulated when cells are stressed by temperatures above the normal physiological range.
They are expressed in unstressed cells also and have a normal function as chaperones, helping other proteins to fold correctly, and are required in much greater amounts if the cell or tissue is stressed by heat.
The increased levels are generated transcriptionally under the influence of a powerful transcription factor, the heat shock factor 1 (HSF1).
The different heat shock proteins were originally named based on their SDS-PAGE mobility, so HSP27 has an an apparent molecular weight of 27kD.
It is an abundant protein even under non-stress conditions and frequently shows up as a major spot on 2 dimensional gels of cells or tissues.
It is known to associate with a variety of other proteins such as actin, intermediate filament subunits and ubiquitin and is found both in the cytoplasm and the nucleus of cells.
HSP27 can become heavily phosphorylated under the influence of multiple protein kinases particularly as a result of activation of the p38/SAPK pathway.
Upregulation of this protein is protective against neurodegenerative diseases at least in certain mouse models (1).
Point mutations in the HSP27 gene are associated with two neurological diseases, Charcot-Marie-Tooth disease type 2F and distal hereditary motor neuropathy IIB (2).
These diseases are associated with axonal loss apparently following defects in the transport of neurofilaments.
The HGNC name for this protein is HSBP1.
Applications:Suitable for use in Immunofluorescence and Western Blot.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1:2000Western Blot: 1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：chicken
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in 10mM sodium azide.
Specificity：Recognizes HSP27 from human, bovine, porcine, mouse, rat and other mammals. Since HSP27 is highly conserved, it is likely that the antibody is effective on other species also.
