84-1239-03　Glial Fibrillary Acidic Protein (GFAP) 100ul　031369

特徴

• Glial Fibrillary Acidic Protein (GFAP) was discovered by Amico Bignami and coworkers as a major fibrous protein of multiple sclerosis plaques (1).
• It was subsequently found to be a member of the 10nm or intermediate filament protein family, specifically the intermediate filament protein family Class III, which also includes peripherin, desmin and vimentin.
• The GFAP protein runs on gels at ~55kDa protein, usually associated with lower molecule weight bands which are thought to be proteolytic fragments and alternate transcripts from the single gene.
• GFAP is strongly and specifically expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves.
• In many damage and disease states GFAP expression is heavily upregulated in astrocytes.
• In addition neural stem cells frequently strongly express GFAP.
• Antibodies to GFAP are therefore very useful as markers of astrocytic cells and neural stem cells.
• In addition many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP.
• Finally, Alexander's disease was recently shown to be caused by point mutations in protein coding region of the GFAP gene (2).
• All forms of Alexander disease are characterized by the presence of Rosenthal fibers, which are GFAP containing cytoplasmic inclusions found in astrocytes.
• The HGNC name for this protein is GFAP.
• Applications:Suitable for use in Immunocytochemistry and Western Blot.
• Other applications not tested.
• Recommended Dilution:Immunocytochemistry: 1:1000 using fluorescent secondary antibodies or 1:5000 using peroxidase or other enzyme linked methodsWestern Blot: 1:50,000 is recommended.
• Expect to see a band at 55kD and another at about 48kD, apparently a breakdown product of the 55kD band
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.