84-1238-98　Coronin 1a (Coronin-1A, CORO1A, CORO1, Coronin-like Protein A, Clipin-A, Coronin-like Protein p57, Tryptophan Aspartate-containing Coat Protein, TACO) 100ul　031354

特徴

• Coronin was originally discovered in Dictyostelium, where it was found to be involved in the chemotactic response of these ameboid cells (1,2).
• The name derives from the fact that the protein is localized at the leading edge or crown of these highly motile cells.
• The name derives from Corona, which is latin for crown.
• Coronin homologues have been found in yeast, C.
• elegans, Drosophila and many other species, and a family them are known in mammals.
• All coronins belong to the WD40 or WD family of proteins, the prototype or which is the beta subunit of trimeric G-proteins.
• The beta subunit proteins all have a beautiful conserved wheel-like seven bladed beta-propeller structure, with each blade being formed by four beta strands generated by one of the WD sequence repeats.
• The G protein beta subunits are believed to function as general purpose binding adapters, mediating numerous regulatory binding interactions between G proteins, G protein coupled receptors and G protein effectors.
• Although sequence analyis suggested that coronin 1a only possess 5 of the these WD repeats, recent structural studies show that, like the G protein beta-subunits, there are seven beta-propellers, generating a compact 7 bladed propellor (3).
• Coronins appear to be particularly involved in binding to actin, actin associated proteins, tubulin and phospholipase C and have been implicated in the mechanisms of chemotaxis and phagocytosis.
• In mammals there are at least five major coronin proteins, named coronins 1 to 5 in one nomenclature.
• Another nomenclature divides these five proteins in coronins 1a and 1b, 2a, 2b and 2c (see the Human Genone Organization Gene Nomenclature Committee link for this family).
• The various coronin proteins have several other alternate names, since they were discovered independently by several different groups.
• The mammalian coronin family members are abundant components of eukaryotic cells, and each type has a restricted cell type specific expression pattern.
• Coronin 1A is the mammalian coronin most similar in protein sequence to the Dictyostelium protein and is found exclusively in hematopoetic lineage cells such as lymphocytes, macrophages and neutrophils.
• The antibody is therefore an excellent marker of cells of this lineage and can also be used to study the leading edges particularly of neutrophils.
• Since the only hematopoetic cells found within the central nervous system are microglia, this antibody is also an excellent marker of this important cell type (4).
• Microglia are numerically fairly minor components of the nervous system, but microglial activation is seen in response to a wide variety of damage and disease states, including ALS, Alzheimer's disease and responses to brain tumors.
• Since coronin 1a is a constitutive component of microglia, the coronin 1a antibody can be used to study both quiescent and activated microglia.
• The HGNC name for this protein is CORO1A.
• Applications:Suitable for use in Immunocytochemistry, Immunofluorescence and Western Blot.
• Other applications not tested.
• Recommended Dilution:Immunocytochemistry (ABC): 1:5000Immunofluorescence: 1:500-1:1000Western Blot: 1:20,000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.