mCherry is derived from proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals), and is used as a fluorescent tracer in trasfection and transgenic experiments.
The prototype for these fluorescent proteins is Green Fluorescent Protein (GFP), which is a ~27kD protein isolated originally from the jellyfish Aequoria victoria.
GFP was the basis of the 2008 Nobel Prize in Chemistry, awarded to Osamu Shimomura, Martin Chalfie and Roger Tsien, specifically for "for the discovery and development of the green fluorescent protein, GFP".
GFP was shown to fluoresce on contact with molecular oxygen, requiring no other cofactors, and so can be expressed in fluorescent form in essentially any prokaryotic or eukaryotic cell.
The mCherry protein is derived from DsRed, a red fluorescent protein related to GFP isolated from so-called disc corals of the genus Discosoma.
DsRed is similar in size and properties to GFP, but, obviously, produces a red rather than a green fluorochrome.
The original DsRed was engineered extensively in the Tsien lab to prevent it from forming tetramers and dimers and to modify and improve the spectral properties.
Several further cycles of mutation, directed modification and evolutionary selection produced mCherry, which has an excitation maximum at 587 nm and and emission maximum at 610nm.
We expressed the mCherry protein sequence shown in reference 4 in bacteria, purified out the mCherry and raised this rabbit polyclonal antibody.
This was affinity purified and was found to stain a band of the expected size in Hek293 cells transfected with the pFin-EF1-mCherry vector designed to express mCherry.
Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:1000.
Band at ~28kDImmunohistochemistry: 1:500Immunofluorescence (IC): 1:500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Grade：Affinity Purified
Purity：Purified by affinity chromatography
Form：Supplied as a liquid in PBS, 5mM sodium azide, 50% glycerol
Specificity：Recognizes mCherry.
Isotype：IgG
Calc Applications Abbrev：IC IF IHC WB
Immunogen：Full-length recombinant protein corresponding to His tagged mCherry purified from E. coli.
