84-1238-87　TrkA (High Affinity Nerve Growth Factor Receptor, Neurotrophic Tyrosine Kinase Receptor Type 1, TRK1 Transforming Tyrosine Kinase Protein, p140-TrkA, Trk-A, NTRK1, TRK) (FITC) 100Tests　029630

特徴

• TrkA is a high affinity nerve growth factor (NGF).
• The Trk proto-oncogene family contains four members, TrkA, TrkB, TrkC, and TrkE, which are variably expressed throughout the central and peripheral nervous systems.
• TrkA binds to nerve growth factor (NGF) and autophosphorylates on tyrosine residues (Tyr490, Tyr674, Tyr675, Tyr751 and Tyr785) to activate multiple downstream effector proteins.
• Phosphorylation at Tyr490 is required for Shc association and subsequent activation of the Ras-MAP kinase-signaling cascade, which leads to activation of Elk-1-dependent gene transcription and neurite growth.
• Phosphorylations at Tyr674 and Tyr675 lie within the catalytic domain of TrkA tyrosine kinase and reflect Trk kinase activity.
• Additionally, phosphorylation at Tyr751 is required for PI3-kinase association and activation of the Akt signaling cascade.
• Applications:Suitable for use in Flow Cytometry.
• Other applications not tested.
• Recommended Dilution:Flow Cytometry: 1:5 with PBS.
• 5-10ul/testsOptimal dilutions to be determined by the researcher.
• Positive Control:HeLa cells
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: FITC conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.