Sirtuin (SIRT5) is known as a nicotinamide adenine dinucleotide- dependent deacetylase that is a member of the silent information regulator 2 (Sir2) family of proteins that is important in aging, metabolism and disease.
SIRT5 is expressed in a variety of tissues.
Two different isoform have been described.
Isoform 1 is reported to be express in the mitochondria and cytoplasm while isoform 2 appears to be localized to the mitochondria only.
SIRT5 has also been linked to many primate-specific functions.
SIRT5 has recently been implicated as having a role in adaptation to fasting, caloric restriction and high protein diets.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5ug/mlImmunohistochemistry: 1:500Optimal dilutions to be determined by the researcher.
Positive Control:HeLa cell lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity：Recognizes human SIRT5. Species Crossreactivity: rat.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Rt
Immunogen：Synthetic linear peptide corresponding to human SIRT5 (KLH). Species sequence homology: chimpanzee and rhesus macaque (100%).
