Wnt/Wingless signaling controls many fundamental processeses, such as cellular growth and fate, and is known to be involved in embryonic segmentation, limb development and CNS patterning.
Wnt signaling is also shown to influence postembryonic regulation of stem cell numbers and stem cell differentiation in epithelia, such as those of the colon and skin
Wnt transduction is mediated by the association of Beta-Catenin with nuclear TCF DNA binding factors, including legless (lgs) and pygopus (pygo).
Whereas lgs enclodes BCL9, pygo encodes a Pygopus (Pygo), a PHD finger protein.
It is reported that BCL9 acts as an adaptor protein that recruits and physically links Pygo to the Beta-Catenin-TCF Complex.
It is demonstrated that the recruitment of Pygo is required for this respective complex to function as a transcriptional co-activator of specific target genes.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Human placenta tissue lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity：Recognizes human Pygo1. Species Crossreactivity: mouse and rat.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic linear peptide corresponding to human Pygopus Homolog 1 (KLH). Species sequence homology: chimpanzee and rhesus macaque (100%).
