84-1238-66　NeuN (Neuronal Nuclei, Neuron Specific Nuclear Protein) (Cy3) 100ul　029556

特徴

• NeuN is a highly characterized and cited mouse monoclonal antibody that specifically recognizes the DNA-binding, neuron-specific protein NeuN, which is present in most CNS and PNS neuronal cell types of all vertebrates tested.
• NeuN protein distributions are apparently restricted to neuronal nuclei, perikarya and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are examples.
• Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuron.
• Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5.
• Strong nuclear staining suggests a nuclear regulatory protein function; however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus.
• No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found.
• Applications:Suitable for use in Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:Immunocytochemistry: 1:100, detected NeuN in rat E18 cortex cells
• Optimal dilutions to be determined by the researcher.
• Positive Control:Rat E18 cortex cells
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Cy conjugates are sensitive to light.