84-1238-63　NEFH (Neurofilament Heavy Polypeptide, NF-H, 200kD Neurofilament Protein, Neurofilament Triplet H Protein, KIAA0845, NFH) 100ug　029551

特徴

• Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm.
• They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules.
• Neurofilaments 10nm in dia.
• are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexes of monomeric proteins.
• The neurofilament triplet proteins 68/70, 160, and 200kD occur in both the central and peripheral nervous system and are usually neuron specific.
• The 68/70kD NF-L protein can self-assemble into a filamentous structure, however the 160kD NF-M and 200kD NF-H proteins require the presence of the 68/70kD NF-L protein to co-assemble.
• Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments.
• Although typically restricted to neurons, neurofilaments have been detected in paragangliomas, adrenal and extra-adrenal pheochromocytomas, carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.1ug/mlImmunohistochemistry: 1ug/mlOptimal dilutions to be determined by the researcher.
• Positive Control:Bovine cerebellum tissue lysate
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.