mRNA capping enzyme is one of the first mRNA transcription cycle factors to be associated with the C-terminal domain and functions by adding an inverted guanylyl group to the 5’ end.
mRNA capping enzyme also has triphosphatase and guanylyltransferase activity.
The CTD also serves to regulate the capping enzyme and has the ability to remove the Ser5 phosphate which is linked to the release of the capping enzyme from the capping complex.
mRNA capping enzyme has a variety of other functions during transcription including; stabilization of polymerase II, activation of early elongation and reduction of re-initiation.
Applications:Suitable for use in Western Blot, Immunofluorescence and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:5000Immunofluorescence (IC): 1:500Optimal dilutions to be determined by the researcher.
Positive Control:HeLa nuclear extract
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity：Recognizes human MRNA. Species Crossreactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：IC IF WB
Calc Crossreactivity：Hu Mo
Immunogen：Recombinant protein corresponding to human MRNA fused to GST-tag.
