Anti-oxidant defense is known to be regulated by various mechanisms including the usage of nuclear factor erythroid 2-related factor 2 (Nrf2).
Under normal physiological conditions, Nrf2 is bound to Kelch-like ECH-associated protein 1 (Keap1), a cytoplasmic repressor.
Keap1 also acts as a substrate adaptor for Cullin3-dependent ubiquitin ligase and also targets Nrf2 for degradation by proteosomes.
The substrate adaptor function of Keap1 is inactivated during physiological stress.
In this respective condition, a range of oxidative and electrophilic stimuli such as ROS, diethyl malonate, and certain disease processes, resulting from Nrf2 accumulation, enters the nucleus and thereby activates the expression of select anti-oxidant genes.
Such genes promote the detoxification of ROS and other harmful molecules, which contribute to the carcinogenesis.
Applications:Suitable for use in Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1ug/mlImmunocytochemistry: 2ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:HeLa cell lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity：Recognizes human KEAP1. Species Crossreactivity: mouse and rat.
Isotype：IgG
Calc Applications Abbrev：IC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic linear peptide corresponding to human KEAP1, conjugated to KLH.
