84-1238-49　INF2 (Inverted Formin-2, HBEBP2-binding Protein C, C14orf151, C14orf173) 100ug　029515

特徴

• NF2 (inverted formin 2) is a mouse formin that has been found to interact with actin through the C-terminus to the formin homology 2 domain.
• The WASP homology 2 motif activates another region that appropriates actin monomers.
• INF2 has a unique ability to speed up polymerization and depolymerization of actin filaments.
• Mutations in the N-terminal diaphanous inhibitory domain of INF2 have been proven to be a main cause of familial focal segmental glomerulosclerosis.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 0.05ug/ml, detected INF2 in 10ug of HeLa cell lysate
• Immunohistochemistry (Paraffin): 1:500, detected INF2 in stomach cancer tissue.
• Requires antigen retrieval using heat treatment prior to staining paraffin sections.
• Sodium citrate buffer, pH 6.0 is recommended
• Optimal dilutions to be determined by the researcher.
• Positive Control:HeLa cell lysate
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.