Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin.
The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each).
Histones are modified post-translationally by the actions of enzymes in both the nucleus and cytoplasm.
These modifications, which include acetylation, phosphorylation, methylation, and ubiquitin-ation, occur predominantly on the N-terminal and C-terminal tails that extend beyond the nucleosome core particle.
Arginine modifications of histones proteins are mediated by two classes of protein arginine methyltransferases (PRMTs), class I and II.
Class I PRMTs place two methyl groups on the one nitrogen atom of the arginine guanidino group, resulting in an asymmetric modification.
In contrast class II PRMTs place two methyl groups on two nitrogen atoms of the guanidino group producing a symmetric dimethylation.
Although there are exceptions, asymmetric modifications are typically associated with transcription activation while symmetric modifications are most commonly associated with transcriptional repression.
Applications:Suitable for use in Western Blot, Dot Blot, Chromatin Immunoprecipitation (ChIP) and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Positive Control:Includes normal rabbit IgG and primers specific for human B-globin
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：25Tests
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide, 30% glycerol.
Specificity：Recognizes human Histone H4. Species Crossreactivity: mouse and rat. Broad species cross-reactivity expected based on sequence similarity.
Isotype：IgG
Calc Applications Abbrev：ChIP DB IC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic linear peptide corresponding to human Histone H4 (KLH).
