Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells.
Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure.
The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes.
These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
Applications:Suitable for use in Western Blot and Chromatin Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Positive Control:Includes normal rabbit serum and primers specific for human B-globin
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：25Tests
Host：rabbit
Source Antibody：human
Grade：Serum
Purity：Serum
Form：Supplied as a liquid in 0.02M phosphate, 0.25M sodium chloride, 0.09% sodium azide, 30% glycerol.
Specificity：Recognizes human Histone H3. Species sequence homology: Mouse, rat, yeast, chicken.
Isotype：IgG
Calc Applications Abbrev：ChIP WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to the C-terminus of human Histone H3 (KLH).
