Forkhead box O (FOXO) transcription factors play an important role in modulating metabolic functions and cell proliferation.
Given the relatively high expression of FOXO1 in insulin-responsive tissues, this transcription factor is highly poised to regulate energy metabolism.
When nutrient and insulin levels are low, FOXO1 promotes expression of gluconeogenic enzymes.
Conversely, in the fed state, insulin levels rise and stimulate uptake of glucose primarily into skeletal muscle and other organs, including adipose tissue.
Under certain pathophysiologic conditions, including insulin resistance, negative signaling to FOXO1 is compromised.
Increased cell proliferation occurs when FOXO1 is inactivated when phosphorylated by Akt at 24aa.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 2ug/ml detected phospho-FOXO1 (Thr24) in HEK293 cell lysate untreated and BMP2-treated
Optimal dilutions to be determined by the researcher.
Positive Control:HEK293 cell lysate untreated and BMP2-treated
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity：Recognizes human FOXO1 phosphorylated at Thr24.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic linear peptide corresponding to human FOXO1 phosphorylated at Thr24 (KLH).
