84-1238-37　FAK, phosphorylated (Tyr397) (Focal Adhesion Kinase 1, FADK 1, pp125FAK, Protein-tyrosine Kinase 2, PTK2, FAK1) 100ul　029472

特徴

• Focal adhesion kinase (FAK) is a non receptor protein tyrosine kinase discovered as a substrate for Src and a key element in integrin signaling.
• FAK plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle.
• FAK regulation includes phosphorylation at multiple tyrosine and serine residues.
• Phosphorylation of tyrosine is generally associated with positive regulation and growth promotion; however, dephosphorylation at these sites occurs as cells enter mitosis (M-Phase).
• In contrast, serine phosphorylation either remains high or is increased as cells enter mitosis and may play a role in focal adhesion disassembly.
• FAK and its phosphorylation states have been implicated in cancer metastasis, tumor cell survival, and adhesion-independent growth.
• Recent evidence indicates that elevation of FAK activity in human carcinoma cells is associated with increased invasive potential.
• A central role in tumor formation and progression suggests that FAK is an attractive target for therapeutic intervention.
• Activation of FAK by integrin clustering leads to autophosphorylation at Tyr397.
• Applications:Suitable for use in Western Blot and Cell Function Assay.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
• Positive Control:Untransfected and Src-transfected RAT2 cell lysates
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.