DEPTOR was recently identified as a novel mTOR-interacting protein whose over-expression in certain cell types represents a new mechanism for activating PI3K/Akt signaling and promoting cell survival.
DEPTOR negatively regulates mTORC1 and mTORC2.
It has been shown that loss of DEPTOR activates various targets that include p70S6K1, Akt, and SGK1, that leads to cell growth and survival.
Little was known about DEPDC6 prior to the discovery of its interactions with mTOR, at which time it was given the new name of DEPTOR.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/ml, detected DEPTOR in HEK293 cell lysate
Optimal dilutions to be determined by the researcher.
Positive Control:HEK293 cell lyaste
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity：Recognizes human DEBTOR.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic linear peptide corresponding to the DEP domain, mTOR-interacting protein of human DEPTOR (KLH). Species sequence homology: Primate (99%), equine (99%), porcine (98%), canine (98%), rat (97%), mouse (96%).
