84-1237-32 RPA32 (Replication Protein A 32kD Subunit, RP-A p32, Replication Factor A Protein 2, RF-A Protein 2, Replication Protein A 34kD Subunit, RP-A p34, RPA2, REPA2, RPA34) (HRP) 100ug 029305
84-1237-32 RPA32 (Replication Protein A 32kD Subunit, RP-A p32, Replication Factor A Protein 2, RF-A Protein 2, Replication Protein A 34kD Subunit, RP-A p34, RPA2, REPA2, RPA34) (HRP) 100ug 029305
Required for DNA recombination, repair and replication.
The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.
Functions as component of the alternative replication protein A complex (aRPA).
aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase.
In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Labeled with horseradish peroxidase (HRP).
Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
HRP conjugates are sensitive to light.
仕様
Size:100ug
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by Protein G affinity chromatography.
Form:Supplied as a liquid in PBS. Labeled with horseradish peroxidase (HRP).
Specificity:Recognizes human RPA32.
Isotype:IgG
Calc Applications Abbrev:E WB
Calc Crossreactivity:Hu
Immunogen:Recombinant corresponding to human RPA32.