Eukaryotic initiation factor 4A plays an important role in the binding of mRNA to the 43S preinitiation complex when protein synthesis begins.
Two highly homologous forms of functional EIF4A genes, Eif4a1 and Eif4a2, have been isolated in mice; yeast cells also possess 2 EIF4A genes, TIF1 and TIF2.
The murine Eif4a and yeast TIF genes appear to belong to a DEAD-box gene family, whose members exhibit extensive aa similarity and contain the asp-glu-ala-asp (DEAD) sequence.
DEAD-box genes have been identified in species ranging from E-coli to humans.
Their function appears to be related to transcriptional/translational regulation (referenced from OMIM).
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Labeled with horseradish peroxidase (HRP).
Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
HRP conjugates are sensitive to light.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in 0.01M PBS, PH 7.4, 0.03% Proclin 300, 50% glycerol. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes human eIF4A2.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Recombinant corresponding to human Eukaryotic initiation factor 4A-II.
