Mouse Spi-6, also known as serpin B9, is a cytoplasmic serine protease inhibitor of ~42kD.
Spi-6 is expressed in dendritic cells, lymphocytes and specific cell types at immune- privileged sites.
It inactivates granzyme B, a natural occurring inducer of programmed cell death.
As such, Spi-6 functions as anti-apoptotic protein.
In cytotxic T cells (CTL), Spi-6 is required to protect clonal bursts (expansion) from granzyme B induced cell death.
However, it is not required for the development of memory cells.
Expression of SPI-6 is of crucial importance in the escape of several tumors from a CTL response and is, therefore, a parameter that influences the feasibility of CTL-mediated immunotherapy of cancer.
Applications:Suitable for use in Western Blot and Flow Cytometry.
Other applications not tested.
Recommended Dilution:Flow Cytometry: 1:50Western Blot: 1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
Specificity：Recognizes mouse Spi-6.
Isotype：IgG
Calc Applications Abbrev：FC WB
Calc Crossreactivity：Mo
Immunogen：Recombinant corresponding to Spi-6 conjugated to GST.
