Ikaros family proteins, characterized by the presence of N-terminal zinc finger DNA-binding domain and C-terminal dimerization domain, belong to the Kruppel transcription factor superfamily.
Ikaros family includes Ikaros, Aiolos, Helios, and possibly EOS and Pegasus.
They can form homodimers as well as heterdimers with other members of the Ikaros family.
Due to differential splicing, multiple isoforms can be generated and some behave in a dominant negative manner upon dimerization.
Ikaros, also known as IKZF1 (Ikaros family zinc finger protein 1) and LYF-1 (Lymphoid transcription factor 1), is expressed abundantly in blood cells.
Genetic studies in mice demonstrate that Ikaros is important for the normal development of B, T, natural killer, and dendritic cells, and that it functions as a tumor suppressor.
Imbalanced expression of different Ikaros isoforms as well as deletions of Ikaros are associated with hematologic malignancies in humans.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:32,000Western Blot: 0.01-0.03ug/ml, observed in lysates of cell line MOLT4 on ~65kD bandsOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity：Recognizes human IKZF1. Species sequence homology: Bovine, canine, mouse, porcine and rat.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Bo Ca Hu Mo Po Rt
Immunogen：Synthetic peptide corresponding to C-DLLRAASENSQDALR, from human IKZF1, at the internal region of the protein (NP_006051.1, NP_001207694.1, NP_001207695.1).
