HE4 (also WDFC2; WAP Four-disulfide core domain protein 2) is a secreted, 25kD glycoprotein member of the Whey Acidic Protein family of molecules.
It is expressed by a wide variety of epithelial cells, including respiratory epithelium, salivary gland mucous cells, breast duct epithelium, distal tubule renal epithelium, and epididymal epithelium.
It is believed to play a role in innate defense, and/or function as a protease inhibitor (perhaps for neutrophil elastase).
Although ovarian cuboidal epithelium does not express HE4, ovarian epithelial tumors do, leading to the suggestion that HE4 could represent a tumor marker for ovarian cancer.
Mature human HE4 is 94aa in length.
It contains two WAP domains that likely mediate antiprotease and/or antimicrobial activity (aa31-73 and 74-123).
There are four potential splice variants.
One shows a deletion of aa27-74, while three others show aa substitutions: 28aa for aa75-124, 23aa for aa1-74, and 10aa for aa71-124.
Over aa31-124, human HE4 shares 63% aa identity with an extended (144aa) mouse form of mature HE4.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:16,000Western Blot: 0.2-1ug/ml, transfected HEK293 transiently expressing full-length Human WFDC2 (myc and DYKDDDDK tagged) on ~25kD bandsOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity：Recognizes human WFDC2. Species sequence homology: Canine.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Ca Hu
Immunogen：Synthetic peptide corresponding to GAEKTGVCPELQADQ, from human WFDC2, at the internal region of the protein (NP_006094.3).
