Vav proteins belong to the Dbl family of guanine nucleotide exchange factors (GEFs) for Rho/Rac small GTPases.
The three identified mammalian Vav proteins (Vav1, Vav2 and Vav3) differ in their expression.
Vav1 is expressed only in hematopoietic cells and is involved in the formation of the immune synapse.
Vav2 and Vav3 are more ubiquitously expressed.
Vav proteins contain the Dbl homology domain, which confers GEF activity, as well as protein interaction domains that allow them to function in pathways regulating actin cytoskeleton organization.
Phosphorylation stimulates the GEF activity of Vav protein towards Rho/Rac.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:128,000Western Blot: 0.3-1ug/ml, observed in human bone marrow lysates on ~80kD bandsOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity：Recognizes human VAV3. Species sequence homology: Canine.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Ca Hu
Immunogen：Synthetic peptide corresponding to CSGEQGTLKLPEK, from human VAV3, at internal region of the protein (NP_006104.4, NP_001073343.1).
