UBE2F (Ubiquitin-conjugating enzyme E2F), a ubiquitously expressed enzyme and member of the ubiquitin-like E2 conjugating enzyme family, which acts as a specific NEDD8 conjugating enzyme, by accepting NEDD8 from the Uba3-NAE1 (APP-BP1/Uba3) E1 complex.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:32,000Western Blot: 0.05-0.2ug/ml, observed in rat pancreas lysates on ~22kD bandsOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity：Recognizes rat UBE2F. Species sequence homology: Bovine, canine, human, mouse and porcine.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Bo Ca Hu Mo Po Rt
Immunogen：Synthetic peptide corresponding to SKLKRDDGLKGSRT-C, from rat UBE2F, at N-terminal (NP_542409.1).
