HIP2 belongs to the ubiquitin-conjugating enzyme family.
It binds selectively to a large region at the N terminus of huntingtin.
This interaction is not influenced by the length of the huntingtin polyglutamine tract.
This protein has been implicated in the degradation of huntingtin and suppression of apoptosis.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:2000Western Blot: 1-3ug/ml, observed in Jurkat cell lysates on ~24kD bandsImmunohistochemistry (Paraffin): 1-2ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity：Recognizes human UBE2K. Species sequence homology: Bovine, canine, mouse and porcine.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Bo Ca Hu Mo Po
Immunogen：Synthetic peptide corresponding to C-SWDVETATELLLSN, from human UBE2K, at C-terminal (NP_005330.1, NP_001104582.1, NP_001104583.1).
