Form a receptor-activated non-selective calcium permeant cation channel.
Acts as a cell-cell contact-dependent endothelial calcium entry channel.
Probably operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors.
Mediates cation entry, with an enhanced permeability to barium over calcium.
May also be activated by intracellular calcium store depletion.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:32,000Western Blot: 0.3-1ug/ml, observed in rat brain lysates on ~110kD bandsOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity：Recognizes rat TRPC4. Species sequence homology: Bovine, canine, human, mouse and porcine.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Bo Ca Hu Mo Po Rt
Immunogen：Synthetic peptide corresponding to C-KHAKEEDSSIDYD, from rat TRPC4, at the internal region (near C-terminal) of the protein (NP_057263.1, NP_003297.1, NP_001129427.1).
