Tissue inhibitors of metalloproteinases (TIMPs) are involved in the control of matrix-metalloproteinase (MMP) activity as a key inhibitor in tissue.
Expression of MMPs and TIMPs are controlled during tissue remodeling to maintain a balance in the regulation of extracellular matrix turnover and other morphogenic processes.
TIMP-2 has also been reported to have growth factor properties.
Zebrafish TIMP-2 shows high similarity to human TIMP-2 with all critical features conserved.
Immunoreactive zebrafish TIMP-2 was detected at the protein level with a recombinant antibody for human TIMP-2.
TIMP-2 was shown to be expressed as early as the one cell stage, and blockage of TIMP-2 translation resulted in abnormal zebrafish development.
Applications:Suitable for use in ELISA.
Other applications not tested.
Recommended Dilution:ELISA: 1:16,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：Zebrafish
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity：Recognizes Timp2. Species sequence homology: Zebrafish.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Ze
Immunogen：Synthetic peptide corresponding to C-TEKIIHGRQSDH, from timp2, at the internal region of the protein (NP_878294.1).
