84-1232-40　TXNRD1 (Thioredoxin Reductase 1, Cytoplasmic, TR, Gene Associated with Retinoic and Interferon-induced Mortality 12 Protein, GRIM-12, Gene Associated with Retinoic and IFN-induced Mortality 12 Protein, KM-102-derived Reductase-like Factor, Thioredoxin Reductase TR1, GRIM12, KDRF) 100ug　T5010-14N

特徴

• The mammalian thioredoxin reductases (TrxRs) are a family of selenocysteine-containing pyridine nucleotide-disulfide oxido-reductases.
• All the mammalian TrxRs are homologous to glutathione reductase with respect to primary structure including the conserved redox catalytic site (-Cys-Val-Asn-Val-Gly-Cys-) but distinctively with a C-terminal extension containing a catalytically active penultimate selenocysteine (SeCys) residue in the conserved sequence(-Gly-Cys-SeCys-Gly).
• TrxR is homodimeric protein in which each monomer includes an FAD prosthetic group, a NADPH binding site and a redox catalytic site.
• Electrons are transferred from NADPH via FAD and the active-site disulfide to C-terminal SeCys-containing redox center, which then reduces the substrate like thioredoxin.
• The members of TrxR family are 55-58 kilodalton in molecular size and composed of three isoforms including cytosolic TrxR1,mitochondrial TrxR2, and TrxR3, known as Trx and GSSG reductase (TGR).
• TrxR plays a key role in protection of cells against oxidative stress and redox-regulatory mechanism of transcription factors and various biological phenomena.
• Applications:Suitable for use in ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:32,000Western Blot: 0.1-1ug/ml, observed in human placenta lysates on ~60kD bandsOptimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.