SPRY1 is a 34-38kD member of the sprouty family of proteins.
It is widely expressed, being found in multiple embryonic and adult tissues.
SPRY1 is considered a negative regulator of cellular signaling.
In particular, it appears to both inhibit MAP kinase signaling following RTK activation, and block TCR signaling following antigen activation.
It interacts with a number of molecules, including PLC-g 1, LAT, CBL, caveolin-1 and SPRY2.
Human SPRY1 is 319aa in length.
It contains one CBL-TKB binding site aa51-57 that is phosphorylated at Tyr53, a Ser-rich region aa112-131, and a Cys-rich domain aa181-306 that mediates intracellular translocation.
SPRY1 undergoes serine phosphorylation, ubiquitination and palmitoylation, the latter which induces SPRY1 to associate with cell membranes.
Over aa1-178, human SPRY1 shares 76% aa identity with mouse SPRY1.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:16,000Western Blot: 1-3ug/mlImmunohistochemistry (Paraffin): 1-3ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity：Recognizes human SPRY1. Species Crossreactivity: Mouse.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to CPSRGQGKPS, from SPRY1, at C-terminal (NP_005832.1, NP_955359.1).
