May contribute to cell volume homeostasis in single cells.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:16,000Western Blot: 0.1-0.3ug/ml, observed in human heart and kidney lysates at ~120kD bandsImmunohistochemistry (Paraffin): 2-4ug/ml Paraffin embedded human heart shows staining of myocardial fibrils in transverse section.
Requires HIER using sodium citrate buffer pH6.0 prior to staining
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:100ug
Host:goat
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity:Recognizes human SLC12A6.
Isotype:IgG
Calc Applications Abbrev:E IHC WB
Calc Crossreactivity:Hu
Immunogen:Synthetic peptide corresponding to SRHIDVCSKTKE, from human SLC12A6, at the internal region of the protein (NP_001035959.1, NP_001035960.1).