Involved in the epithelial to mesenchymal transition (EMT) and formation and maintenance of embryonic mesoderm By similarity.
Binds to 3 E-boxes of the E-cadherin gene promoter and represses its transcription.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:128,000Western Blot: 0.1-0.3ug/ml, observed in lysates of cell line A431 and human kidney lysate on ~28kD bandsOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity：Recognizes human SNAI1. Species Crossreactivity: Mouse. Species sequence homology: Rat.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to RKPSDPNRKPNY-C, from SNAI1, at N-terminal (NP_005976.2).
