Binds differentially to the SH3 domains of certain proteins of signal transduction pathways.
This protein binds preferentially to the ABL1 proto-oncogene, SRC and GRB2.
Shows GAP activity for Rac-related proteins but not for Rho- or Ras-related proteins.
It inhibits PDGF-induced membrane ruffling mediated by Rac.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:16,000Western Blot: 1-3ug/ml, observed in human lung and kidney lysates on ~70kD bandsImmunohistochemistry (Paraffin): 0.3ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity：Recognizes human SH3BP1.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to C-PQPRPRSLASETN, from human SH3BP1, at C-terminal (NP_061830.3).
