Ubiquitin-like molecules (UBLs), such as SUMO1 (UBL1), are structurally related to ubiquitin and can be ligated to target proteins in a similar manner as ubiquitin.1,2 However, covalent attachment of UBLs does not result in degradation of the modified proteins..
Like ubiquitin, UBLs are synthesized as precursor proteins, with 1 or more aa following the C-terminal glycine-glycine residues of the mature UBL protein.
Thus, the tail sequences of the UBL precursors need to be removed by UBL-specific proteases such as SUMO1-specific protease 1 (SUSP1) prior to their conjugation to target proteins.
Applications:Suitable for use in ELISA.
Other applications not tested.
Recommended Dilution:ELISA: 1:16,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity：Recognizes SENP6. Species sequence homology: Canine, human and mouse.
Isotype：IgG
Calc Applications Abbrev：E
Calc Crossreactivity：Ca Hu Mo
Immunogen：Synthetic peptide corresponding to AAGKSGGSAGEIT-C, from SENP6, at N-terminal (NP_056386.2, NP_001093879.1).
