Members of the semaphorin protein family are involved in axonal guidance during neural development.
Semaphorin 5a is thought to have a bifunctional role in axon guidance, exerting both attractive and inhibitory effects on developing axons associated with limbic function.
The thrombospondin repeats of Semaphorin 5a physically interact with both heparan sulfate proteoglycans and chondroitin sulfate proteoglycans, inducing attractive and inhibitory guidance cues, respectively.
The biological responses to Semaphorin 5a are induced through binding to its high-affinity receptor, plexin B3, triggering intracellular signaling through Met.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:ELISA: 1:128,000Western Blot: 0.03ug/mlImmunohistochemistry (Paraffin): 4-6ug/ml paraffin embedded human kidney shows membranous and vesicular staining in PCT cells
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Specificity：Recognizes human Semaphorin 5A.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Bo Ca Hu Mo Rt
Immunogen：Synthetic peptide corresponding to DEATKKACYSKGKS, from the internal region of human Semaphorin 5A. Species Sequence Homology: bovine, canine, mouse and rat
