Roundabout Homolog 1 (ROBO1) is a type I transmembrane protein belonging to the ROBO family, which defines a novel subfamily of immunoglobulin superfamily proteins.
ROBO family proteins are evolutionarily conserved receptors that interact with the secreted Slit proteins.
ROBO-Slit interactions play an important role in axon guidance across the midline during central nervous system development.
ROBO signaling also has a role in tumor angiogenesis and immune responses.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:8000Western Blot: 1-3ug/ml, observed in human endothelial cell line HUVEC lysates on ~250kD bandsOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity：Recognizes human ROBO1. Species sequence homology: Bovine, canine, mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Bo Ca Hu Mo Rt
Immunogen：Synthetic peptide corresponding to C-GDVDLSNKINEMK, from hman ROBO1, at the internal region of the protein (NP_002932.1, NP_598334.1).
