TRIM69 is a member of the RING-B-box-coiled-coil (RBCC) family with an N-terminal RING finger motif, a PRY domain and a C-terminal SPRY domain.
The mouse ortholog of TRIM69 is specifically expressed in germ cells at the round spermatid stages during spermatogenesis and, when overexpressed, induces apoptosis.
Applications:Suitable for use in ELISA, Immunohistochemistry and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:32,000Western Blot: 0.5-2ug/mlImmunohistochemistry (Paraffin): 2.5-3.8ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:100ug
Host:goat
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity:Recognizes RNF36. Species Crossreactivity: human and mouse. Species sequence homology: Bovine, canine, porcine and rat.
Isotype:IgG
Calc Applications Abbrev:E IHC WB
Calc Crossreactivity:Bo Ca Hu Mo Po Rt
Immunogen:Synthetic peptide corresponding to C-GENKEPLHILHPQ, from RNF36, at C-terminal (NP_892030.3, NP_542783.2).