This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases.
This kinase contains 2 non-identical kinase catalytic domains and phosphorylates several residues of the S6 ribosomal protein.
The kinase activity of this protein leads to an increase in protein synthesis and cell proliferation.
Amplification of the region of DNA encoding this gene and overexpression of this kinase are seen in some breast cancer cell lines.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:128,000Western Blot: 0.2-1ug/ml, observed in human skeletal muscle and placenta lysates on ~65-70kD bandsImmunohistochemistry (Paraffin): 1-2ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity：Recognizes human RPS6KB1. Species sequence homology: Bovine, canine, mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Bo Ca Hu Mo Rt
Immunogen：Synthetic peptide corresponding to C-MISKRPEHLRMNL, from human RPS6KB1, at C-terminal (NP_003152.1).
