Importin 8 is a 1037aa nuclear protein with a RAN binding site.
It belongs to RAN-binding protein super family and hence shares an N-terminal sequence motif with importin-beta that appears to account for RAN GTP binding.
It binds to the nuclear pore complex (NPC) and is well known as a transport carrier that mediates nuclear import of ribosomal proteins with a classical nuclear localization signal.
Importin 8 works by binding to the nuclear pore complex and, along with RANGTP and RANBP1, inhibits the GAP stimulation of the RAN GTPase.
Along with transportin it also helps in the nuclear import of SRP19.
Applications:Suitable for use in ELISA and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:32,000Immunohistochemistry (Paraffin): 4-6ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity：Recognizes human IPO8. Species sequence homology: Bovine, canine and porcine.
Isotype：IgG
Calc Applications Abbrev：E IHC
Calc Crossreactivity：Bo Ca Hu Po
Immunogen：Synthetic peptide corresponding to C-SAFNFGTVPSNN, from IPO8, at C-terminal (NP_006381.2, NP_001177924.1).
