Mediates the nuclear export of proteins (cargos) with broad substrate specificity.
In the nucleus binds cooperatively to its cargo and to the GTPase Ran in its active GTP-bound form.
Docking of this trimeric complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins.
Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the cargo from the export receptor.
XPO7 then return to the nuclear compartment and mediate another round of transport.
The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.
Applications:Suitable for use in ELISA and Western Blot Other applications not tested.
Recommended Dilution:ELISA: 1:32,000Western Blot: 0.5-1ug/ml, observed in human testis and Hela lysates on ~110kD bandsOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity：Recognizes human XPO7. Species sequence homology: Bovine, canine, mouse and porcine.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Bo Ca Hu Mo Po
Immunogen：Synthetic peptide corresponding to C-NSTYGVNSNDMMS, from human XPO7, at C-terminal (NP_001093631.1, NP_055839, NP_001093632.1).
