Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair.
The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex.
Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER).
The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates.
The 9-1-1 complex is necessary for the recruitment of C12orf32/RHINO to sites of double-stranded breaks (DSB) occurring during the S phase.
RAD9A possesses 3'->5' double stranded DNA exonuclease activity.
Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:32,000Western Blot: 0.3-1ug/ml, observed in lysates of cell lines A431 and Daudi on ~70kD bandsOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity：Recognizes human RAD9A. Species sequence homology: Canine and mouse.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Ca Hu Mo
Immunogen：Synthetic peptide corresponding to C-QGPSPVLAEDSEGE, from human RAD9A, at C-termina (NP_004575.1).
