Rho GTPases control a variety of cellular processes.
There are 3 subtypes of Rho GTPases in the Ras superfamily of small G proteins: RHO (see MIM 165370), RAC (see RAC1; MIM 602048), and CDC42 (MIM 116952).
GTPase-activating proteins (GAPs) bind activated forms of Rho GTPases and stimulate GTP hydrolysis.
Through this catalytic function, Rho GAPs negatively regulate Rho-mediated signals.
GAPs may also serve as effector molecules and play a role in signaling downstream of Rho and other Ras-like GTPases.[supplied by OMIM]
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:32,000Western Blot: 1-3ug/mlImmunohistochemistry (Paraffin): 1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity：Recognizes human RACGAP1. Species sequence homology: Bovine and canine.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Bo Ca Hu
Immunogen：Synthetic peptide corresponding to C-GRQGNFFASPMLK, from human RACGAP1, at C-terminal (NP_037409.2, NP_001119576.1, NP_001119575.1).
