OCT1 is a transcription factor that binds the octamer motif 5'-ATTTGCAT-3' in promoters of various genes.OCT1 contains one homeobox DNA binding domain and is part of the POU transcription factor family due to the presence of one POU domain, a highly charged motif that selectively binds the octamer motif.OCT1 is also known as POU domain class 2 transcription factor, octamer-binding transcription factor, oct-1, OTF-1, NF-A1, and POU2F1.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:4000Western Blot: 0.5-2ug/ml, nuclear lysates of cell line HeLa observed on ~100kD bandsOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity：Recognizes human POU2F1. Species sequence homology: Bovine, canine, mouse, porcine and rat.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Bo Ca Hu Mo Po Rt
Immunogen：Synthetic peptide corresponding to C-NVQSKSNEESGDSQQ, from human POU2F1, at the internal region, near N-terminal (NP_002688.2).
