Plexin D1 is a type I transmembrane protein that is the prototype of the Plexin D subfamily in the Plexin family.
It is expressed on multiple tissues including fetal neuronal and endothelial cells.
Plexin D1 expression is also induced on tumor vasculature and tumor cells.
The 1879aa mature human Plexin D1 contains a large (1225aa) extracellular domain with a 500aa sema domain and three short MRS (Met-related sequences) cysteine-rich motifs.
By alternative splicing, multiple Plexin-D1 isoforms may exist.
Plexin D1 binds Sema4A to suppress angiogenesis.
It also binds Sema3E directly to mediate repulsive vascular vessal guidance.
In mouse, this interaction has been shown to be independent of Neuropilin and Sema3A.
Over the extracellular region used as immunogen, human Plexin D1 shares 89% and 91% aa identity with the mouse and canine protein, respectively.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:ELISA (Peptide): 1:128,000Western Blot: 0.5ug/ml detects a band at ~250kD in human arterial endothelial cell lysates.
Immunohistochemistry: Stains paraffin-embedded mouse brain after epitope retrieval in citrate buffer, pH 6.0
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity：Recognizes human PLXND1. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to C-LAEPKKSHRQSH, from PLXND1, at the internal region of human Plexin D1 (NP_055918.2). Species Sequence Homology: canine, porcine and bovine
