PHLPP2, a protein phosphatase and negative regulator, which mediates the inactivation of Akt and protein kinase C (PKC) activity, through the direct dephosphorylation of key regulatory sites: AKT1 (Ser473), PRKCA (Ser657) and PRKCB isoform beta-II (Ser660).
PHLPP2, and its alternative isoform PHLPP1, have been implicated as important suppressors of tumor cell growth, being linked with breast, ovarian and colon cancer.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:32,000Western Blot: 0.1-0.3ug/ml, observed in human brain (amygdala, hippocampus, substantia nigra) lysates on ~140kD bandsOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity：Recognizes human PHLPPL.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to C-PHEEDRTEPPEEFD, from human PHLPPL, at C-terminal (NP_055835.2).
