84-1228-66　PEPCK2 (Phosphoenolpyruvate Carboxykinase [GTP], Mitochondrial, PEPCK-M, Phosphoenolpyruvate Carboxylase, PCK2) 100ug　P3240-04B

特徴

• This gene encodes a member of the phosphoenolpyruvate carboxykinase (GTP) family.
• The protein is a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of GTP.
• A cytosolic form encoded by a different gene has also been characterized and is the key enzyme of gluconeogenesis in the liver.
• The encoded protein may serve a similar function, although it is constitutively expressed and not modulated by hormones such as glucagon and insulin that regulate the cytosolic form.
• Alternatively spliced transcript variants have been described.
• Applications:Suitable for use in ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:1000Western Blot: 0.1-0.3ug/ml, observed in human liver lysates and in lysates of cell line HepG2 on ~70kD bandsOptimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.