Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antigen-presenting cells (APC).
T-cell activation is generally self-limited as activated T cells express receptors such as PDCD-1 that mediate inhibitory signals from the APC.
PDCD-1 can bind two different but related ligands, PDL-1 and PDL-2.
PDL-1 is a B7-related protein that inhibits cell-mediated immune responses by reducing the secretion of IL-2 and IL-10 from memory T cells.
This suggests that PDL-1 may be useful in reducing allogenic CD4+ memory T-cell responses to endothelial cells, thereby reducing the likelihood of host immune responses to allografts.
At least two isoforms of PDL-1 are known to exist; this antibody is specific to the larger isoform.
PDL-1 antibody has no cross-reactivity to PDL-2.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:8000Western Blot: 0.3-1ug/ml, observed in human heart lysates on ~35kD bandsImmunohistochemistry (Paraffin): 4-6ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity：Recognizes human CD247.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to CKKQSDTHLEET, from human CD274, at C-terminal (NP_054862.1).
