Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals.
The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle.
Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation.
(provided by RefSeq).
Tissue specificity: Expressed predominantly in the heart.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:1000Western Blot: 1-3ug/ml, observed in rat heart lysates on ~65-70kD bandsOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity：Recognizes human PDK1. Species Crossreactivity: Rat. Species sequence homology: Bovine, canine, mouse and porcine.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Bo Ca Hu Mo Po Rt
Immunogen：Synthetic peptide corresponding to C-DFKDKSAEDAK, from PDK1, at the internal region of the protein (NP_002601.1).
![PDK1 (PDHK1, [Pyruvate Dehydrogenase [Lipoamide]] Kinase Isozyme 1, Mitochondrial, Pyruvate Dehydrogenase Kinase Isoform 1) 100ug](https://aimg.as-1.co.jp/c/84/1228/59/85133520.jpg)