Pax-5 is a 46kD, B cell specificactivator protein (BSAP).
In the early stages of B cell developmemt, Pax-5 influences the expression of several B-cell-specific genes, such as CD19 and CD20.
Pax-5 is expressed primarily in pro-, pre-, and mature B cells, but not in plasma cells.
Interestingly, Pax-5 mRNA is transiently detected in the mesencephalon and spinal cord during embryogenesis.
Expression then shifts to the fetal liver and correlates with the onset of B lymphopoiesis.
This indicates that Pax-5 is important in B cell development, but may also have a role in proper neuronal development.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:64,000Western Blot: 0.3-1ug/ml, human lymph and human spleen lysates observed on ~40kD bandsOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity：Recognizes human PAX5.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to DLEKNYPTPRTSR-C, from human PAX5, at N-terminal (NP_057953.1). Species sequence homology: mouse
