The P2X7 ATP receptor has a distinctive long C-terminal tail with multiple potential protein and lipid interaction motifs and is highly polymorphic.
It is a cation selective ion channel that opens up on binding of extracellular ATP.
Sustained activation by extracellular ATP results in the formation of a reversible pore in the plasma membrane that is permeable to hydrophilic solutes of up to 900Da.
Once a pore is opened massive upset of cytoplasmic ion homeostasis occurs and the pore stays open as long as it is bound by ATP.
Should ATP stimulation continue the cell will become irreversibly damaged and die.
P2X7 plays a key role in the maturation and release of IL-1 and other IL-1 family members during inflammation.
As such, P2X7 blockers might be useful as anti-inflammatory agents.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:16,000Western Blot: 1-3ug/ml, observed in human brain (frontal cortex) lysates on ~37, 70kD bandsImmunohistochemistry (Paraffin): 3-6ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity：Recognizes human P2RX7. Species sequence homology: Canine, mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Ca Hu Mo Rt
Immunogen：Synthetic peptide corresponding to YETNKVTRIQSMNY-C, from human P2RX7, at N-terminal (NP_002553.2).
