Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate.
Not required for the proliferation of neural progenitor cells before the onset of neurogenesis.
Also involved postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity.
May also mediate local repair of brain ventricular wall damage.
Applications:Suitable for use in ELISA, Immunocytochemistry, Immunofluorescence and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:32,000Western Blot: 0.03-0.1ug/ml, mouse brain lysates observed on ~80kD bandsOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：goat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity：Recognizes mouse NUMB. Species sequence homology: Bovine, canine and human.
Isotype：IgG
Calc Applications Abbrev：E IC IF WB
Calc Crossreactivity：Bo Ca Hu Mo
Immunogen：Synthetic peptide corresponding to C-PFSSDLQKTFEIEL, from mouse NUMB, at C-terminal (NP_001005743.1, 001005744.1, NP_003735, NP_001005745.1).
